Protein captured on CM5 Chip via anti-his antibody can bind Mouse EGF, Human Fc Tag, in SPR assay (Biacore T200) with an affinity constant of 9.96 nM.
Purity
>95% (by Tris-Bis PAGE and HPLC).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Mouse EGFR Protein (C-terminal His Tag) (A331561)
Recombinant Mouse EGFR Protein (C-terminal His Tag) (A331561) binding to Recombinant Mouse EGFR Protein (C-terminal His Tag) (A331561) on a CM5 Chip via Anti-His Antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 9.96 nM.
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