The protein has a predicted molecular mass of 28.4 kDa after removal of the signal peptide. The apparent molecular mass of GPRC5D-mFc is approximately 25-35 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
The mouse Fc tag consists of amino acids Pro99-Lys330.
Synonyms
G protein coupled receptor family C group 5 member D, G-protein coupled receptor family C group 5 member D, GPC5D_HUMAN, GPRC5D
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human GPCR GPRC5D Protein (Fc Tag) (A318179)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human GPCR GPRC5D Protein (Fc Tag) (A318179) which can bind Anti-GPCR GPRC5D Antibody [DM91] - Azide free (A318614) in a linear range of 0.17-22.45 µg/ml.
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