Full-length transmembrane human GPCR GPRC5D protein expressed in exosome membranes.
Applications
TEM, ELISA
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human GPCR GPRC5D protein has a MW of 38.6 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Reconstitute with distilled sterile water.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
G protein coupled receptor family C group 5 member D, G-protein coupled receptor family C group 5 member D, GPC5D_HUMAN, GPRC5D
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Exosome Human GPCR GPRC5D Protein (A318487)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Exosome Human GPCR GPRC5D Protein (A318487). Serial diluted Anti-GPCR GPRC5D Antibody [DM90] - Azide free (A318616) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-GPCR GPRC5D Antibody [DM90] - Azide free (A318616) binding with GPRC5D exosomes is 41.99 µg/ml.