Immobilized Recombinant human TNFRSF11B (2 µg/mL) bind Recombinant human TNFSF11 in a functional ELISA with a linear range of 2-8 ng/mL. Inhibition of the TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL: ED50 = 28.5-114 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human Osteoprotegerin Protein (C-terminal His Tag) (A331132)
ELISA examination of immobilized Recombinant human TNFRSF11B (2 µg/mL) binding Recombinant Human Osteoprotegerin Protein (C-terminal His Tag) (A331132): linear range = 2-8 ng/mL.
Recombinant Human Osteoprotegerin Protein (C-terminal His Tag) (A331132)
TRAIL-mediated cytotoxicityin L-929 mouse fibroblast cells treated with TRAIL inhibited by Recombinant Human Osteoprotegerin Protein (C-terminal His Tag) (A331132): ED50 = 28.5-114 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
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