Immobilized Recombinant Human TNFSF11 (2 µg/mL) bind Recombinant Mouse TNFRSF11B in a functional ELISA with a linear range of 3-10 ng/mL. Inhibition of the TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL: ED50 = 0.13-0.54 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Purity
>87% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Mouse Osteoprotegerin Protein (C-terminal Human Fc and His Tag) (A331886)
ELISA examination of immobilized Recombinant Human TNFSF11 (2µg/mL) binding Recombinant Mouse Osteoprotegerin Protein (C-terminal Human Fc and His Tag) (A331886): linear range = 3-10 ng/mL.
Recombinant Mouse Osteoprotegerin Protein (C-terminal Human Fc and His Tag) (A331886)
TRAIL-mediated cytotoxicityin L-929 mouse fibroblast cells treated with TRAIL inhibited by Recombinant Mouse Osteoprotegerin Protein (C-terminal Human Fc and His Tag) (A331886): ED50 = 0.13-0.54 ng/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
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