Immobilized Human TNFRSF1A (2 µg/mL) bind Mouse TNFa in a functional ELISA with a linear range of 4-135 ng/mL. Inhibition of the TNFa-mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D: ED50 = typically 22-88ng/mL in the presence of 0.25 ng/mL recombinant human TNFa.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CD120a, p55, p60, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFRSF1A, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human TNFR1 Protein (C-terminal His Tag) (A331343)
ELISA examination of immobilized recombinant Human TNFRSF1A (2 µg/mL) binding Recombinant Human TNFR1 Protein (C-terminal His Tag) (A331343): linear range = 4-135 ng/mL.
Recombinant Human TNFR1 Protein (C-terminal His Tag) (A331343)
TNFa-mediated cytotoxicityin L-929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D inhibited by Recombinant Human TNFR1 Protein (C-terminal His Tag) (A331343): ED50 = typically 22-88 ng/mL in the presence of 0.25 ng/mL Recombinant human TNFa.
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