Immobilized Mouse TNF-alpha Protein (0.5 µg/mL) bind Mouse TNFR1 in a functional ELISA with a linear range of 0.06-15.7 ng/mL. Inhibition of the the TNFa-mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D: ED50 = typically 75-300 ng/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-a.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CD120a, p55, p60, TNF-R1, TNF-RI, TNFAR, TNFR-I, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Mouse TNFRSF1A Protein (C-terminal Human Fc and His Tag) (A331894)
TNFa-mediated cytotoxicityin L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D inhibited by Recombinant Mouse TNFRSF1A Protein (C-terminal Human Fc and His Tag) (A331894): ED50 = typically 75-300 ng/mL in the presence of 0.1 ng/mL of Recombinant mouse TNF-a.
Standard Curve - Recombinant Mouse TNFRSF1A Protein (C-terminal Human Fc and His Tag) (A331894)
ELISA examination of immobilized Mouse TNF-alpha Protein (0.5 µg/mL) binding Recombinant Mouse TNFRSF1A Protein (C-terminal Human Fc and His Tag) (A331894): linear range = 0.06-15.7 ng/mL.
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