Reconstitute with 0.2 ml of distilled water. For long term storage the addition of 0.1% Bovine Serum Albumin is recommended. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Endotoxin Level
< 0.1 ng/µg
Storage
Store undiluted at -20°C only, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Tumor necrosis factor alpha (TNFa) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.
Sandwich ELISA analysis of murine TNF alpha using Anti-TNF alpha Antibody [MP6-XT3] (A281255) as a monoclonal capture antibody and biotinylated Rabbit anti Mouse TNF alpha as a detection antibody with Streptavidin:HRP as the tertiary reagent for the detection of the antigen; Recombinant Mouse TNF alpha Protein (A320506) at the concentrations indicated on the x-axis
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