Reconstitute with 0.2 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Endotoxin Level
< 0.1 ng/µg
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells - this batch demonstrated an ED50 of <0.05ng/ml.