Synthetic Membrane Nanoparticle Human CD20 Protein
Description
Full-length transmembrane human CD20 protein expressed on the cell surface of membrane nanoparticles.
Applications
ELISA, FACS
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CD20 protein has a MW of 33.1 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Synthetic Membrane Nanoparticle Human CD20 Protein (A318472)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Membrane Nanoparticle Human CD20 Protein (A318472). Serial diluted Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) binding with CD20 full length membrane nanoparticles is 128.8 µg/ml.
Synthetic Membrane Nanoparticle Human CD20 Protein (A318472)
FACS analysis of Synthetic Membrane Nanoparticle Human CD20 Protein (A318472): A. Negative Control 1: Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained only with Goat Anti-Human lgG Antibody (Alexa Fluor 488). B. Negative Control 2: Control membrane nanoparticles samples were stained with Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). C. Negative Control 3: Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained with Anti-CCR8 Antibody (an irrelevant antibody) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488). D. Synthetic Membrane Nanoparticle Human CD20 Protein (A318472) samples were stained with Anti-CD20 Humanized Antibody [Obinutuzumab Biosimilar] - Azide free (A318814) at 2 µg/ml, followed by Goat Anti-Human lgG Antibody (Alexa Fluor 488).
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