Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human Claudin 6 protein to be purified and analysed in vitro.
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Tag
N-terminal MBP Tag and C-terminal Flag Tag
Predicted MW
63.3 kDa
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 with 5% - 8% Trehalose. Do not use solvents with pH lower than 6.5 in subsequent experiments.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synthetic Nanodisc Human Claudin 6 Protein (A332832)
Immobilised Synthetic Nanodisc Human Claudin 6 Protein at 0.2 µg/well can bind serial diluted IMAB027 Biosimilar - Anti-Claudin 6 Antibody (A318880). After washing and incubation with a secondary antibody the EC50 for IMAB027 Biosimilar - Anti-Claudin 6 Antibody (A318880) binding with Synthetic Nanodisc Human Claudin 6 Protein is 723.6ng/ml.
Synthetic Nanodisc Human Claudin 6 Protein (A332832)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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