Synthetic Virus-like Particle Human Claudin 6 Protein
Description
Full-length transmembrane human Claudin 6 protein expressed on the cell surface of Virus like particles.
Applications
ELISA
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human Claudin 6 protein has a MW of 23 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Reconstitute with distilled sterile water.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synthetic Virus-like Particle Human Claudin 6 Protein (A318460)
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Virus-like Particle Human Claudin 6 Protein (A318460). Serial diluted Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-Claudin 6 Humanized Antibody [IMAB027] - Azide free (A318880) binding with CLDN6-VLP is 5.616 µg/ml.
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