Goat Anti-Mouse IgG H&L Antibody (CF®405S) - Highly Cross-Adsorbed (A343988)

MCF-7 cells stained with rabbit anti-COX IV followed by CF®405S goat anti-rabbit to label mitochondria (blue), and mouse anti-phosphorylated RNA polymerase II followed by CF®647 goat anti-mouse to label nuclei (red).

This chart summarizes commonly used CF® dyes grouped by their suitability for specific imaging modalities, including alternative spectra applications, four-color confocal imaging, near-infrared western blotting, photoacoustic imaging, STED, SIM, STORM, TIRF, and two-photon microscopy. Each dye is shown with its characteristic excitation and emission wavelengths (nm), providing a practical reference for selecting spectrally compatible dyes and optimizing multicolor experimental design across a range of fluorescence techniques.

Normalized emission spectra of the CF® dye family spanning the visible to near-infrared range are shown, illustrating the spectral diversity and overlap between dyes. Curves represent relative fluorescence intensity as a function of emission wavelength (nm), with peak positions corresponding to each dye’s characteristic emission maximum. This reference highlights the broad coverage of CF® dyes for multicolor fluorescence applications and aids in selecting compatible dye combinations for imaging, flow cytometry, and other fluorescence-based assays.

CF®405S produces brighter antibody conjugates than Alexa Fluor® 405. Flow cytometry analysis was performed in the Pacific Blue® detection channel on a BD LSRII cytometer using Jurkat cells stained with mouse anti-CD3 or an isotype control, followed by goat anti-mouse secondary antibodies conjugated to either Alexa Fluor® 405 or CF®405S at varying degrees of labeling. Bars represent geometric mean fluorescence intensity.