This product recognizes the heavy and light chains of Rabbit IgG.
Applications
ICC/IF, IHC, WB, Flow Cytometry, ELISA
Clonality
Polyclonal
Isotype
IgG
Conjugate
HRP
Product Form
Supplied as a liquid; 1 mg vials are lyophilized.
Concentration
1 mg/mL
Formulation
Liquid: Supplied in phosphate buffered saline (PBS) containing 50% glycerol, 2 mg/ml BSA, and 0.05% sodium azide. Lyophilized: Supplied in phosphate Buffered Saline with 20 mg/ml BSA and 20 mg/ml trehalose.
Storage
Shipped at +4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Looking for a specific protein conjugate to simplify your workflow? We offer a library of over 2,000 targets conjugated to your choice of CF® dye. To enquire about a custom product, contact us directly.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Representative example of multiplex tyramide signal amplification labeling in human colon FFPE tissue using an HRP-conjugated secondary antibody. Cytokeratin (pan) was detected with CF®488A tyramide, highlighting the cytoskeletal compartment in green, Histone H1 was visualized with Cyanine 555 tyramide to mark nuclei in red, and ZO1 was labeled with CF®640R tyramide to delineate tight junctions in magenta. All primary antibodies were mouse monoclonal antibodies, and signal development was achieved using an HRP-conjugated goat anti-mouse secondary antibody. Staining was performed sequentially, with antibody stripping by microwaving between each labeling cycle. The image shown is representative of results obtained with the HRP-conjugated secondary antibody.
Publishing research using Goat Anti-Rabbit IgG H&L Antibody (HRP), Cross-Adsorbed (A343882)? Please let us know so that we can list the citation on this page.
