Goat anti-rabbit IgG Fc (FITC) secondary antibody.
Host Species
Goat
Target Species
Rabbit
Specificity
This antibody recognises the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.Goat anti rabbit IgG antibody has been used successfully as a secondary detection reagent for rabbit primary antibodies using immunohistochemical techniques.
Applications
Flow Cytometry
Recommended Dilutions
Flow Cytometry: 1:200 - 1:2,000
Clonality
Polyclonal
Isotype
IgG
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Product Form
Liquid
Concentration
1 mg/ml
Formulation
Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.Goat anti rabbit IgG antibody has been used successfully as a secondary detection reagent for rabbit primary antibodies using immunohistochemical techniques (Redondo et al. 2013).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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