Anti-CBL Antibody [ARC59894] (A309299)

Name: Anti-CBL Antibody [ARC59894]
Brand: Antibodies.com
SKU: A309299

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This product is discontinued

Anti-CBL Antibody [ARC59894] (A309299) has been discontinued and is no longer available.

View all Anti-CBL Antibodies.

Specifications

Name

Anti-CBL Antibody [ARC59894]

Description

Rabbit monoclonal [ARC59894] antibody to CBL.

Applications

WB, IHC, IP

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:100-1:500, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of Human CBL.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59894

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4732447J05Rik, C CBL, Cas Br M (murine) ecotropic retroviral transforming sequence, Casitas B lineage lymphoma proto oncogene, Casitas B-lineage lymphoma proto-oncogene, CBL 2, CBL2, CBL_HUMAN, E3 ubiquitin protein ligase CBL, E3 ubiquitin-protein ligase CBL, Oncogene CBL2, Proto oncogene c CBL, Proto-oncogene c-CBL, RGD1561386, RING finger protein 55, RNF55, Signal transduction protein CBL

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CBL Antibody [ARC59894] (A309299)

Western blot analysis of various lysates, using Anti-CBL Antibody [ARC59894] (A309299) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-CBL Antibody [ARC59894] (A309299)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-CBL Antibody [ARC59894] (A309299) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CBL Antibody [ARC59894] (A309299)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-CBL Antibody [ARC59894] (A309299) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-CBL Antibody [ARC59894] (A309299)

Immunoprecipitation analysis of 600µg extracts of rat testis cells using 3µg of Anti-CBL Antibody [ARC59894] (A309299). This Western blot was performed on the immunoprecipitate using Anti-CBL Antibody [ARC59894] (A309299) at a dilution of 1:1000.

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