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Anti-CD14 Antibody [ARC54507] (A308988)

Western Blot - Anti-CD14 Antibody [ARC54507] (A308988) - Antibodies.com Immunohistochemistry - Anti-CD14 Antibody [ARC54507] (A308988) - Antibodies.com
This product is discontinued

Anti-CD14 Antibody [ARC54507] (A308988) has been discontinued and is no longer available.

View all Anti-CD14 Antibodies.

Name
Anti-CD14 Antibody [ARC54507]
Description
Rabbit monoclonal [ARC54507] antibody to CD14.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-352 of human CD14 (NP_000582.1).
Sequence
TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPAC
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC54507
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
50 - 60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD14 Antibody [ARC54507] (A308988) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD14 Antibody [ARC54507] (A308988)
Western blot analysis of various lysates, using Anti-CD14 Antibody [ARC54507] (A308988) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120s.
Immunohistochemistry - Anti-CD14 Antibody [ARC54507] (A308988) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD14 Antibody [ARC54507] (A308988)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD14 Antibody [ARC54507] (A308988) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-CD14 Antibody [ARC54507] (A308988)? Please let us know so that we can list the citation on this page.

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