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Anti-CD14 Antibody [MEM-18] (A85710)

Western Blot - Anti-CD14 Antibody [MEM-18] (A85709) - Antibodies.com
$195
Product Datasheet

Mouse monoclonal [MEM-18] antibody to CD14 for Flow Cytometry, IP, WB and ELISA.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-CD14 Antibody [MEM-18]
Description
Mouse monoclonal [MEM-18] antibody to CD14.
Specificity
This antibody reacts with CD14, a 53-55 kDa GPI-linked extracellular membrane glycoprotein expressed on monocytes, macrophages, and weakly on granulocytes. It is also expressed by most tissue macrophages. In human, the epitope recognized by this antibody is located between amino acids 57-64.
Applications
Flow Cytometry, IP, WB, ELISA
Dilutions
ELISA: This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100), Flow Cytometry: 4 µg/ml, WB: Non-reducing conditions.
Reactivity
Human, Non-Human Primates
Immunogen
A crude mixture of human urinary proteins, precipitated by ammonium sulphate, from the urine of a patient suffering from proteinuria.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-18
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
53-55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD14 Antibody [MEM-18] (A85709) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD14 Antibody [MEM-18] (A85710)
Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using Anti-CD14 Antibody (A85710). Lane 1: Whole cell lysate HEK 293 transfected with empty vector. Lane 2: Tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA. Lane 3: Whole cell lysate of HEK 293 transfected with human CD14 cDNA.
Publishing research using Anti-CD14 Antibody [MEM-18] (A85710)? Please let us know so that we can list the citation on this page.

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