This antibody recognises the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage , a soluble form of CD14 also exists. CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner. Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:2 Pack Size: 0.1 mg 1:10 Pack Size: 25 µg, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Reactivity

Human, Dog, Goat, Cat, Rabbit, Mink, Bovine, Pig, Sheep, Cynomolgus monkey, Llama

Host

Mouse

Clonality

Monoclonal

Clone ID

TÜK4

Isotype

IgG2a

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti human CD14 antibody, clone TÜK4 recognizes the human CD14 cell surface antigen. CD14 is a ~55 kDa glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986).CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria.Mouse anti human CD14 antibody, clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Use of the anti-human CD14 antibody, Low Endotoxin format is recommended for this purpose.

Synonyms

CD 14, CD14 antigen, CD14 molecule, CD14_HUMAN, CD_antigen=CD14, LPS-R, Mo2, Monocyte differentiation antigen CD14, Monocyte differentiation antigen CD14 urinary form, Monocyte differentiation antigen CD14, membrane-bound form, Myeloid cell specific leucine rich glycoprotein, Myeloid cell-specific leucine-rich glycoprotein

Isotype Controls