This antibody recognises CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Reactivity

Mouse

Immunogen

HEK293F cell expressing CD16 and gamma chain.

Host

Rat

Clonality

Monoclonal

Clone ID

AT154-2

Isotype

IgG2b

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and <0.1% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

Synonyms

CD 16, CD 16a, CD16a, CD16a antigen, CD16B, CD16b antigen, Fc fragment of IgG, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG low affinity IIIa receptor (CD16), Fc fragment of IgG receptor IIIa, Fc fragment of IgG, low affinity III, receptor (CD16), Fc fragment of IgG, low affinity III, receptor for (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for, Fc fragment of IgG, low affinity IIIb, receptor (CD16b), Fc fragment of IgG, low affinity IIIb, receptor for (CD16), Fc gamma R3, Fc gamma receptor III 2 (CD 16), Fc gamma receptor III A, Fc gamma receptor IIIA, Fc gamma receptor IIIb (CD 16), Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIII beta, Fc gamma RIIIa, Fc gamma RIIIb, Fc of IgG, Fc-gamma receptor III2 (CD 16), Fc-gamma receptor III2 (CD16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG 3, FCG3, FCG3A_HUMAN, FCgammaRIIIA, FCGR 3, FCGR 3A, FCGR3, FCGR3A, FCGR3A protein, FCGRIII, FCGRIII-2, FcR 10, FcR-10, FcR10, FcRIII, FcRIIIa, IGFR 3, IGFR3, IgG Fc receptor III 1, IgG Fc receptor III 2, IgG Fc receptor III-2, IMD20, immunoglobulin G Fc receptor III, immunoglobulin G Fc receptor III-2, Low affinity IIIa receptor, Low affinity immunoglobulin gamma Fc region receptor III A, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor IIIB, neutrophil-specific antigen NA

Isotype Controls