Anti-CD16 Antibody [AT154-2] (A279712)

Anti-CD16 Antibody [AT154-2]

Rat monoclonal [AT154-2] antibody to CD16.

This antibody recognises CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10, IHC-Fr: 1:100 - 1:1,000, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Mouse

HEK293F cell expressing CD16 and gamma chain.

Rat

Monoclonal

AT154-2

IgG2b

1 mg/ml

Liquid

Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD16 anitbody, clone AT154-2, recognizes CD16, also known as FcRIII. CD16 is a low affiniity activatory receptor which interacts preferentially with mouse IgG1, IgG2a and IgG2b immune complexes and is involved in various effector functions including phagocytosis, NK antibody dependent cellular cytotoxicity and the release of inflammatory mediators.In mice, CD16 is expressed on macrophages, NK cells, granulocytes, myeloid precursors and subpopulations of T lymphocytes.

CD16-II, CD16a, CD16a antigen, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG3, FcgammaRIIIA, FCGR3, FCGR3A, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A