This antibody recognises human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages , whereas CD16B is primarily expressed by granulocytes. Clone KD1 recognises both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine and ovine.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reactivity

Human, Bovine, Sheep, Dolphin

Cross Reactivity

This antibody does not cross react with Rat or Dog

Immunogen

A polyclonal population of NK cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

KD1

Isotype

IgG2a

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).

Synonyms

CD 16, CD 16a, CD16a, CD16a antigen, CD16B, CD16b antigen, Fc fragment of IgG, Fc fragment of IgG low affinity IIIa receptor, Fc fragment of IgG low affinity IIIa receptor (CD16), Fc fragment of IgG receptor IIIa, Fc fragment of IgG, low affinity III, receptor (CD16), Fc fragment of IgG, low affinity III, receptor for (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16), Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG, low affinity IIIa, receptor for, Fc fragment of IgG, low affinity IIIb, receptor (CD16b), Fc fragment of IgG, low affinity IIIb, receptor for (CD16), Fc gamma R3, Fc gamma receptor III 2 (CD 16), Fc gamma receptor III A, Fc gamma receptor IIIA, Fc gamma receptor IIIb (CD 16), Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIII beta, Fc gamma RIIIa, Fc gamma RIIIb, Fc of IgG, Fc-gamma receptor III2 (CD 16), Fc-gamma receptor III2 (CD16), Fc-gamma receptor IIIb (CD16), Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG 3, FCG3, FCG3A_HUMAN, FCgammaRIIIA, FCGR 3, FCGR 3A, FCGR3, FCGR3A, FCGR3A protein, FCGRIII, FCGRIII-2, FcR 10, FcR-10, FcR10, FcRIII, FcRIIIa, IGFR 3, IGFR3, IgG Fc receptor III 1, IgG Fc receptor III 2, IgG Fc receptor III-2, IMD20, immunoglobulin G Fc receptor III, immunoglobulin G Fc receptor III-2, Low affinity IIIa receptor, Low affinity immunoglobulin gamma Fc region receptor III A, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor IIIB, neutrophil-specific antigen NA

Isotype Controls