Anti-IL-2 Receptor alpha Antibody [9.14] (A278703)

Anti-IL-2 Receptor alpha Antibody [9.14]

Mouse monoclonal [9.14] antibody to IL-2 Receptor alpha.

This antibody recognises the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Ra), a single pass type I membrane protein expressed by activated ovine T-cells.Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132). A non-covalent association of the a and ß subunits is required to form the high affinity receptor for IL-2. Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Ra in other species including humans (Verhagen et. al.1993).Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011). Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Sheep

Mouse

Monoclonal

9.14

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Sheep CD25, clone 9.14 recognizes the ovine homologue of human CD25, also known as interleukin-2 receptor alpha chain (IL-2Ra), a single pass type I membrane protein expressed by activated ovine T-cells.Ovine Interleukin-2 is a cytokine involved in the proliferation, growth and differentiation of T-cells, B-cells and NK cells and its receptor is composed of 3 subunits, an a chain (CD25), a ß chain (CD122) and a ? chain (CD132). A non-covalent association of the a and ß subunits is required to form the high affinity receptor for IL-2.Mouse anti sheep CD25, clone 9.14 immunoprecipitates a band of ~47 kDa under reducing conditions, as expected for the mature protein due to high glycosylation, consistent with the observed molecular weight of IL-2Ra in other species including humans (Verhagen et. al.1993).Antibodies to CD4, FoxP3 and CD25 may be used elucidate properties of T regulatory cells (T-regs), a unique subset of T helper cells that function in the control of effector cells vital in preventing autoimmunity (Rocchi et. al. 2011).Mouse anti sheep CD25, clone 9.14 is one of a wide range of monoclonal antibodies available from Bio-Rad for ovine research and provides an important tool for the identification of ovine CD25.