Recombinant rabbit monoclonal [DM53] antibody to CD276.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM53
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
Flow cytometry data of serially titrated Anti-CD276 Antibody [DM53] - Azide free (A318643) on on Expi 293 cell line transfected with human B7-H3. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Expi 293 cell line transfected with irrelevant protein (A) and human B7-H3 (B) were surface stained with Anti-CD276 Antibody [DM53] - Azide free (A318643) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
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