Recombinant humanized monoclonal [Enoblituzumab Biosimilar] antibody to CD276.
Applications
ELISA, Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze / thaw cycles.
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human CD276 Protein (Fc Chimera 6xHis Tag) (A318396) can bind Anti-CD276 Humanized Antibody [Enoblituzumab Biosimilar] - Azide free (A318946) in a linear range of 0.24-31.25 µg/ml.
HEK293 cell line transfected with irrelevant protein (B) and human B7-H3 (A) were surface stained with Anti-CD276 Humanized Antibody [Enoblituzumab Biosimilar] - Azide free (A318946) at 1 µg/ml followed by Anti-Human IgG Antibody (Alexa 488).
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