Anti-CD3 Antibody [1F4] (A279103)

Anti-CD3 Antibody [1F4]

Mouse monoclonal [1F4] antibody to CD3.

This antibody recognises rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

IHC-P, IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10 - 1:25, IHC-Fr: 1:10 - 1:25, IHC-P: 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.

Mouse

Monoclonal

1F4

IgM

Unconjugated

Ammonium sulphate precipiation of tissue culture supernatant.

1 mg/ml

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at 4°C.

Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a ~25 kDa antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.

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