Anti-CD31 Antibody [ARC5013-19] (A305350)

Name: Anti-CD31 Antibody [ARC5013-19]
Brand: Antibodies.com
SKU: A305350

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This product is discontinued

Anti-CD31 Antibody [ARC5013-19] (A305350) has been discontinued and is no longer available.

View all Anti-CD31 Antibodies.

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A86230

Anti-CD31 Antibody [MEM-05]

Mouse monoclonal [MEM-05] antibody to CD31 for Flow Cytometry, WB, IHC-P and ICC.

A86230

Anti-CD31 Antibody [MEM-05]

Mouse monoclonal [MEM-05] antibody to CD31 for Flow Cytometry, WB, IHC-P and ICC.

Specifications

Name

Anti-CD31 Antibody [ARC5013-19]

Description

Rabbit monoclonal [ARC5013-19] antibody to CD31.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_000433.4).

Sequence

AKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC5013-19

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 - 140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350)

Western blot analysis of extracts of various cell lines, using Anti-CD31 Antibody [ARC5013-19] (A305350) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-CD31 Antibody [ARC5013-19] (A305350)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-CD31 Antibody [ARC5013-19] (A305350)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-CD31 Antibody [ARC5013-19] (A305350). This Western blot was performed on the immunoprecipitate using Anti-CD31 Antibody [ARC5013-19] (A305350) at a dilution of 1:1000.

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