Anti-CD44 Antibody [25.32] (A278730)

Anti-CD44 Antibody [25.32]

Mouse monoclonal [25.32] antibody to CD44.

This antibody recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation. Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017).

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Sheep, Bovine, Goat, Human

Sheep efferent lymphatic duct lymphocytes.

Mouse

Monoclonal

25.32

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Sheep CD44 antibody, clone 25.32 recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation.Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017).

BA-1, CD 44, CD44 antigen, CD44 molecule, CD44 molecule (Indian blood group), CD44_HUMAN, CDw44, Cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HSA, HUTCH-I, HUTCH1, Hyaluronate receptor, IN, INLU-related p80 Glycoprotein, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP-1, PGP-I, PGP1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, Soluble CD44