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Anti-CD44 Antibody [ARC52411] (A305401)

Western Blot - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.com Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.com Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.com Immunofluorescence - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC52411] antibody to CD44 for WB, IHC, ICC/IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-CD44 Antibody [ARC52411]
Description
Rabbit monoclonal [ARC52411] antibody to CD44.
Applications
WB, IHC, ICC/IF, Flow Cytometry
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:100-1:500, ICC/IF: 1:500-1:1,000, Flow Cytometry: 0.2µg per 106 cells in 100µl volume
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3).
Sequence
AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC52411
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
82 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BA-1, CD 44, CD44 antigen, CD44 molecule, CD44 molecule (Indian blood group), CD44_HUMAN, CDw44, Cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HSA, HUTCH-I, HUTCH1, Hyaluronate receptor, IN, INLU-related p80 Glycoprotein, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP-1, PGP-I, PGP1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, Soluble CD44
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD44 Antibody [ARC52411] (A305401)
Western blot analysis of extracts of HeLa cells, using Anti-CD44 Antibody [ARC52411] (A305401) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10S.
Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401)
Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-CD44 Antibody [ARC52411] (A305401) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD44 Antibody [ARC52411] (A305401)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD44 Antibody [ARC52411] (A305401) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD44 Antibody [ARC52411] (A305401) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD44 Antibody [ARC52411] (A305401)
Immunofluorescence analysis of HeLa using Anti-CD44 Antibody [ARC52411] (A305401) at a dilution of 1:1,000 (40x lens). DAPI was used to stain the cell nuclei (blue).
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