Anti-CD5 Antibody [ARC1633] (A307625)

Name: Anti-CD5 Antibody [ARC1633]
Brand: Antibodies.com
SKU: A307625

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This product is discontinued

Anti-CD5 Antibody [ARC1633] (A307625) has been discontinued and is no longer available.

View all Anti-CD5 Antibodies.

Specifications

Name

Anti-CD5 Antibody [ARC1633]

Description

Rabbit monoclonal [ARC1633] antibody to CD5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD5 (P06127).

Sequence

MPMGSLQPLATLYLLGMLVASCLGRLSWYDPDFQARLTRSNSKCQGQLEVYLKDGWHMVCSQSWGRSSKQWEDPSQASKVCQRLNCGVPLSLGPFLVTYT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1633

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD 5, CD5 antigen, CD5 antigen (p56 62), CD5 molecule, CD5_HUMAN, LEU1, LyA, Lymphocyte antigen T1/Leu-1, OTTHUMP00000236973, T-cell surface glycoprotein CD5, T1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CD5 Antibody [ARC1633] (A307625)

Western blot analysis of extracts of Jurkat cells, using Anti-CD5 Antibody [ARC1633] (A307625) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-CD5 Antibody [ARC1633] (A307625)

Immunohistochemistry analysis of paraffin-embedded human spleen using Anti-CD5 Antibody [ARC1633] (A307625) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-CD5 Antibody [ARC1633] (A307625)

Immunofluorescence analysis of Jurkat cells using Anti-CD5 Antibody [ARC1633] (A307625) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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