This antibody recognizes an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Applications
Flow Cytometry, IP, WB, IHC-Fr, ELISA
Dilutions
ELISA: This antibody has been tested as the detection antibody in a sandwich ELISA with the capture antibody MEM-32, Flow Cytometry: 1 µg/ml; Positive Control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line, WB: Laurylmaltoside lysing buffer; Non-reducing conditions: 1-2 µg/ml.
Reactivity
Human
Immunogen
Stimulated human leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CRIS1
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.