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Anti-CD99 Antibody (A8988)

Western Blot - Anti-CD99 Antibody (A8988) - Antibodies.com Immunohistochemistry - Anti-CD99 Antibody (A8988) - Antibodies.com Immunohistochemistry - Anti-CD99 Antibody (A8988) - Antibodies.com Immunofluorescence - Anti-CD99 Antibody (A8988) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD99 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD99 Antibody
Description
Rabbit polyclonal antibody to CD99.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 23-185 of human CD99 (NP_002405.1).
Sequence
DGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADAPGVIPGIVGAVVVAVAGAISSFIAYQKKKLCFKENAEQGEVDMESHRNANAEPAVQRTLLEK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
22 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
12E7, Antigen identified by monoclonal 12E7, Y homolog, Antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigen, CD99 molecule, CD99_HUMAN, Cell surface antigen 12E7, Cell surface antigen HBA 71, Cell surface antigen O13, E2 antigen, HBA71, MIC 2X, MIC 2Y, MIC2, MIC2 (monoclonal, MIC2X, MIC2Y, MSK5X, Protein MIC2, Surface antigen MIC2, T cell surface glycoprotein E2, T-cell surface glycoprotein E2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD99 Antibody (A8988) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD99 Antibody (A8988)
Western blot analysis of extracts of various cell lines, using Anti-CD99 Antibody (A8988) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunohistochemistry - Anti-CD99 Antibody (A8988) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD99 Antibody (A8988)
Immunohistochemistry analysis of paraffin-embedded human islet using Anti-CD99 Antibody (A8988) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD99 Antibody (A8988) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD99 Antibody (A8988)
Immunohistochemistry analysis of paraffin-embedded human testis using Anti-CD99 Antibody (A8988) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-CD99 Antibody (A8988) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-CD99 Antibody (A8988)
Immunofluorescence analysis of Jurkat cells using Anti-CD99 Antibody (A8988) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD99 Antibody (A8988)? Please let us know so that we can list the citation on this page.

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