Anti-Cdc25A Antibody (A13349)

Name: Anti-Cdc25A Antibody
Brand: Antibodies.com
SKU: A13349
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Cdc25A for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Cdc25A Antibody

Description

Rabbit polyclonal antibody to Cdc25A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 86-328 of CDC25A (NP_001780.2).

Sequence

LDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALKRSHSDSLDHDIFQLIDPDENKENEAFEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSAPARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPKESTNPEKAHETLHQSLSLASSPKGTIEN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdc 25a, CDC25A2, CDC25A2 CAG isoform, Cell division cycle 25 homolog A (S. pombe), Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, D9Ertd393e, Dual specificity phosphatase Cdc25A, M phase inducer phosphatase 1, M-phase inducer phosphatase 1, MGC115549, MPIP1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Cdc25A Antibody (A13349)

Western blot analysis of extracts of various cell lines, using Anti-Cdc25A Antibody (A13349) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Cdc25A Antibody (A13349)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Cdc25A Antibody (A13349) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cdc25A Antibody (A13349)

Immunohistochemistry analysis of paraffin-embedded human liver damage using Anti-Cdc25A Antibody (A13349) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cdc25A Antibody (A13349)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Cdc25A Antibody (A13349) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.