Anti-CDK1 Antibody [ARC50611] (A305461)

Name: Anti-CDK1 Antibody [ARC50611]
Brand: Antibodies.com
SKU: A305461

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This product is discontinued

Anti-CDK1 Antibody [ARC50611] (A305461) has been discontinued and is no longer available.

View all Anti-CDK1 Antibodies.

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A86650

Anti-CDK1 Antibody [POH-1]

Mouse monoclonal [POH-1] antibody to CDK1 for IP, WB, IHC-P and ICC.

A86650

Anti-CDK1 Antibody [POH-1]

Mouse monoclonal [POH-1] antibody to CDK1 for IP, WB, IHC-P and ICC.

Specifications

Name

Anti-CDK1 Antibody [ARC50611]

Description

Rabbit monoclonal [ARC50611] antibody to CDK1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CDK1 (NP_001777.1).

Sequence

ATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50611

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdc 2, Cdc2, CDC28A, CDK 1, CDK1_HUMAN, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-CDK1 Antibody [ARC50611] (A305461)

Western blot analysis of various lysates, using Anti-CDK1 Antibody [ARC50611] (A305461) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CDK1 Antibody [ARC50611] (A305461)

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Figure 3: Immunohistochemistry - Anti-CDK1 Antibody [ARC50611] (A305461)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-CDK1 Antibody [ARC50611] (A305461) at a dilution of 1:100(40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CDK1 Antibody [ARC50611] (A305461)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-CDK1 Antibody [ARC50611] (A305461) at a dilution of 1:100(40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.