Anti-CDK2 Antibody (A91675)

Name: Anti-CDK2 Antibody
Brand: Antibodies.com
SKU: A91675
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to CDK2 for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody Centrosome Marker

Highlighted products for Anti-CDK2 Antibody

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A96410

Anti-CDK2 Antibody

Rabbit polyclonal antibody to CDK2 for WB, IF and ELISA.

(7)

A96410

Anti-CDK2 Antibody

Rabbit polyclonal antibody to CDK2 for WB, IF and ELISA.

Specifications

Name

Anti-CDK2 Antibody

Description

Rabbit polyclonal antibody to CDK2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:100-1:500, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-298 of human CDK2 (NP_001789.2).

Sequence

RALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

33 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2_HUMAN, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-CDK2 Antibody (A91675)

Western blot analysis of extracts of various cell lines, using Anti-CDK2 Antibody (A91675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-CDK2 Antibody (A91675)

Western blot analysis of extracts from normal (control) and CDK2 knockout (KO) 293T cells, using Anti-CDK2 Antibody (A91675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-CDK2 Antibody (A91675)

Western blot analysis of extracts of HeLa cells, using Anti-CDK2 Antibody (A91675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-CDK2 Antibody (A91675)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CDK2 Antibody (A91675) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.