Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Name: Anti-CDK2 (phospho Thr160) Antibody [ARC57167]
Brand: Antibodies.com
SKU: A309005

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This product is discontinued

Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) has been discontinued and is no longer available.

View all Anti-CDK2 Antibodies.

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A96410

Anti-CDK2 Antibody

Rabbit polyclonal antibody to CDK2 for WB, IF and ELISA.

(7)

A96410

Anti-CDK2 Antibody

Rabbit polyclonal antibody to CDK2 for WB, IF and ELISA.

Specifications

Name

Anti-CDK2 (phospho Thr160) Antibody [ARC57167]

Description

Rabbit monoclonal [ARC57167] antibody to CDK2 (phospho Thr160).

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:6,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T160 of human Phospho-CDK2-T160 (NP_001789.2).

Sequence

TYTHE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57167

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Cdc2 related protein kinase, cdc2-related protein kinase, CDC28, CDC2A, Cdk 2, CDK1, CDK2_HUMAN, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, cyclin dependent kinase 2-alpha, Cyclin-dependent kinase 2, kinase Cdc2, MPF, p33 protein kinase, p33(CDK2)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of HeLa, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Western blot analysis of C6, using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at 1:5,000 dilution. C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CDK2 (phospho Thr160) Antibody [ARC57167] (A309005) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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