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Anti-Clathrin heavy chain Antibody (A88620)

Western Blot - Anti-Clathrin heavy chain Antibody (A88620) - Antibodies.com Immunofluorescence - Anti-Clathrin heavy chain Antibody (A88620) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Clathrin heavy chain for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Clathrin heavy chain Antibody
Description
Rabbit polyclonal antibody to Clathrin heavy chain.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1451-1675 of human Clathrin heavy chain (NP_004850.1).
Sequence
YLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
191 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CHC17, Clathrin heavy chain 1, Clathrin heavy chain 2, Clathrin heavy chain like 1, Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide, CLH 17, CLH 22, CLH-17, CLH17, CLH1_HUMAN, CLH22, CLTC, CLTCL, CLTCL1, CLTCL2, CLTD, Hc, KIAA0034
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Clathrin heavy chain Antibody (A88620) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Clathrin heavy chain Antibody (A88620)
Western blot analysis of extracts of various cell lines, using Anti-Clathrin heavy chain Antibody (A88620) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-Clathrin heavy chain Antibody (A88620) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Clathrin heavy chain Antibody (A88620)
Immunofluorescence analysis of HeLa cells using Anti-Clathrin heavy chain Antibody (A88620) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Clathrin heavy chain Antibody (A88620)? Please let us know so that we can list the citation on this page.

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