Anti-Clathrin heavy chain Antibody [ARC1228] (A308642)

Name: Anti-Clathrin heavy chain Antibody [ARC1228]
Brand: Antibodies.com
SKU: A308642
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1228] antibody to Clathrin heavy chain for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Mitochondrial Marker

Specifications

Name

Anti-Clathrin heavy chain Antibody [ARC1228]

Description

Rabbit monoclonal [ARC1228] antibody to Clathrin heavy chain.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Clathrin heavy chain (Q00610).

Sequence

SFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1228

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

192 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CHC17, Clathrin heavy chain 1, Clathrin heavy chain 2, Clathrin heavy chain like 1, Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide, CLH 17, CLH 22, CLH-17, CLH17, CLH1_HUMAN, CLH22, CLTC, CLTCL, CLTCL1, CLTCL2, CLTD, Hc, KIAA0034

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Clathrin heavy chain Antibody [ARC1228] (A308642)

Western blot analysis of extracts of various cell lines, using Anti-Clathrin heavy chain Antibody [ARC1228] (A308642) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Clathrin heavy chain Antibody [ARC1228] (A308642)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Clathrin heavy chain Antibody [ARC1228] (A308642) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Clathrin heavy chain Antibody [ARC1228] (A308642)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Clathrin heavy chain Antibody [ARC1228] (A308642) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Clathrin heavy chain Antibody [ARC1228] (A308642)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Clathrin heavy chain Antibody [ARC1228] (A308642) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.