Anti-Clusterin Antibody [ARC52367] (A308825)

Name: Anti-Clusterin Antibody [ARC52367]
Brand: Antibodies.com
SKU: A308825

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This product is discontinued

Anti-Clusterin Antibody [ARC52367] (A308825) has been discontinued and is no longer available.

View all Anti-Clusterin Antibodies.

Specifications

Name

Anti-Clusterin Antibody [ARC52367]

Description

Rabbit monoclonal [ARC52367] antibody to Clusterin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:10,000-1:80,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 314-449 of human Clusterin alpha chain (NP_001822.3).

Sequence

STNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52367

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 - 42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

40, AAG 4, AAG4, Aging associated protein 4, Aging-associated gene 4 protein, AI893575, APO J, Apo-J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, ApolipoproteinJ, CLI, CLU, CLU1, CLU2, Clusterin alpha chain, Clusterin beta chain, CLUS_HUMAN, Complement associated protein SP 40, Complement associated protein SP 40 40, Complement associated protein SP40, Complement cytolysis inhibitor, Complement cytolysis inhibitor a chain, Complement cytolysis inhibitor b chain, Complement lysis inhibitor, Complement-associated protein SP-40, D14Ucla3, Dimeric acid glycoprotein, Glycoprotein 80, Glycoprotein III, GP80, Ku70-binding protein 1, KUB 1, KUB1, MGC24903, NA1/NA2, RATTRPM2B, SGP 2, SGP2, SP 40, SP40, Sugp-2, Sulfated glycoprotein 2, Testosterone repressed prostate message 2, Testosterone-repressed prostate message 2, TRPM 2, TRPM-2, TRPM2, TRPM2B, Trpmb

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Clusterin Antibody [ARC52367] (A308825)

Western blot analysis of extracts of various cell lines, using Anti-Clusterin Antibody [ARC52367] (A308825) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Clusterin Antibody [ARC52367] (A308825)

Western blot analysis of extracts of Mouse plasma, using Anti-Clusterin Antibody [ARC52367] (A308825) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 45s.

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Figure 3: Immunohistochemistry - Anti-Clusterin Antibody [ARC52367] (A308825)

Immunohistochemistry analysis of paraffin-embedded human tonsil (72900 times dilution of primary antibody) using Anti-Clusterin Antibody [ARC52367] (A308825)Immunohistochemistry analysis of paraffin-embedded human tonsil (72900 times dilution of primary antibody) using Clusterin alpha chain antibody ( A21104) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Clusterin Antibody [ARC52367] (A308825)

Immunohistochemistry analysis of paraffin-embedded human tonsil (24300 times dilution of primary antibody) using Anti-Clusterin Antibody [ARC52367] (A308825)Immunohistochemistry analysis of paraffin-embedded human tonsil (24300 times dilution of primary antibody) using Clusterin alpha chain antibody ( A21104) at a dilution of 1:70000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.