Rabbit polyclonal antibody to ADD1 (Phospho-Ser726)
Specificity
The antibody detects endogenous level of ADD1only when phosphorylated at serine 726.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from Hela cells using ADD1 (Phospho-Ser726) Antibody #11182 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Phospho-Ser726) Antibody #11182 (left) or the same antibody preincubated with blocking peptide (right).
