Rabbit polyclonal antibody to Akt (Phospho-Ser473)
Specificity
The antibody detects endogenous level of Akt only when phosphorylated at serine 473.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
60kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473) Antibody #11054 (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pre-treated with U0126and LY294002 cells using Akt (Phospho-Ser473) Antibody#11054.
![Immunohistochemistry - Anti-AKT Antibody [RM316] (A121372) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121394_1.png?profile=product_alternative)
