Rabbit polyclonal antibody to AKT1 (phospho-Thr450)
Specificity
The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450) Antibody #11502 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-AKT1 Antibody [AKT1/2784] (A248468) - Antibodies.com](https://cdn.antibodies.com/image/catalog/248/A248468_1.jpg?profile=product_alternative)
