This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of MW 44 kDa) which is a critical costimulatory receptor of T cells.
Applications
Flow Cytometry, IP, WB, IHC-Fr, ICC
Dilutions
Flow Cytometry: 1-12 µg/ml
Reactivity
Human, Non-Human Primates
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA.
Host
Mouse
Clonality
Monoclonal
Clone ID
CD28.2
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD28 with Anti-CD28 Antibody [CD28.2] (A86493), (concentration in sample 1,6 µg/ml) GAM APC.
Western blotting analysis of human CD28 using Anti-CD28 Antibody [CD28.2] (A86493) on lysates of Jurkat cells and THP1 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD28 was detected around 35-40 kDa.
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