Rabbit polyclonal antibody to Cdc25B (Phospho-Ser149)
Specificity
The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1.0 mg/mL
Predicted MW
62kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149) Antibody #11553.
Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody #11553.
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