Rabbit polyclonal antibody to Cyclin B1 (phospho-Ser147)
Specificity
The antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Phospho-Ser147) Antibody #11540 (left) or the same antibody preincubated with blocking peptide (right).
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (A285847) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285848_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] (A250460) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250460_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] - BSA and Azide free (A253640) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253640_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] (A250459) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250459_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] - BSA and Azide free (A253639) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253639_1.jpg?profile=product_alternative)
