Rabbit polyclonal antibody to eIF2a (Phospho-Ser51)
Specificity
The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.
Applications
ELISA, WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat, Pig, Canine, Fish
Immunogen
Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
38kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody #11279 (left) or the same antibody preincubated with blocking peptide #51279 (right).
