Mouse monoclonal [MEM-267] antibody to HLA DR (PE).
Specificity
This antibody specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (i.e. APC: dendritic cells, B lymphocytes, monocytes, macrophages).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml, Stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.
Reactivity
Human
Immunogen
Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-267
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow Cytometry - Anti-HLA DR Antibody [MEM-267] (PE) (A85929)
Flow cytometry surface staining pattern of human peripheral whole blood labeling HLA DR with Anti-HLA DR Antibody [MEM-267] (PE) (A85929), (concentration in sample 9 µg/ml).
Flow Cytometry - Anti-HLA DR Antibody [MEM-267] (PE) (A85929)
Flow cytometry multicolor surface staining of human peripheral whole blood stained using Anti-HLA DR Antibody [MEM-267] (PE) (A85929), (concentration in sample 9 µg/ml) and Anti-HLA DR Antibody [L243] (APC) (A86732), (10 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-HLA DR Antibody [MEM-267] (PE) (A85929)
Separation of human HLA-DR1 (empty) positive HLA-DR positive cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling HLA DR with Anti-HLA DR Antibody [MEM-267] (PE) (A85929), (concentration in sample 9 µg/ml).
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