Rabbit polyclonal antibody to ICAM-1 (Phospho-Tyr512)
Specificity
The antibody detects endogenous level of ICAM-1 only when phosphorylated at tyrosine 512.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
89 92kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ICAM1, Intercellular adhesion molecule 1, Major group rhinovirus receptor
Western blot analysis of extracts from HUVEC cells using ICAM-1 (Phospho-Tyr512) Antibody #11083 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody #11083.
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