Rabbit polyclonal antibody to MAP3K8 (Phospho-Ser400)
Specificity
The antibody detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.
Applications
IHC
Dilutions
Immunohistochemistry: 1:50~1:100
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 400(C-Q-S(p)-L-D) derived from HumanMAP3K8.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cancer Osaka thyroid oncogene, COT, ESTF, Mitogen-activated protein kinase kinase kinase 8, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, TPL-2, Tumor progression locus 2
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (Phospho-Ser400) antibody #11739 (left)or the same antibody preincubated with blocking peptide (right).
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