Anti-MEK1 (Phospho-Ser221) Antibody (A50192)

Anti-MEK1 (Phospho-Ser221) Antibody

Rabbit polyclonal antibody to MEK1 (Phospho-Ser221)

The antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.

WB, IHC, ICC/IF

Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200

Human, Mouse, Rat

Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1.

Rabbit

Polyclonal

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1.0 mg/mL

45kd

Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1